Hey you! Were you thinking, I wish I could just predict all my TMT spectra so I didn’t have to search them every time? ME TOO!
PROSIT now does deep learning prediction of TMT labeled spectra.
WHAT??? I know!!
How to do it? Okay, well this worked for me. I basically just followed this old tutorial I made:
Then I opened the EncyclopeDIA output file and made a new column (or row, I still don’t know the difference) and I think I just put in HCD in every entry, saved it and loaded it to Prosit.
I made the data an MSPepSearch compatible file and loaded it into a copy of PD where the free software developed by NIST wasn’t disabled due to there being no money being funneled to a big company for that particular PC!